Florida Highway Patrol said Telisa Brooks, 49, was riding her bicycle on US 19 when she was struck by a truck.
The driver of the truck, Roger Williams, Jr. of Georgia, was traveling south on US 19 when he veered off the road onto the shoulder, striking Brooks.
Authorities said Brooks was pronounced dead at the scene. According to officials, Williams was taken to the hospital with blood pressure problems.
Officials said the northbound lanes of US 19 were shut down most of the morning.
FHP said the accident is still under investigation and Williams may face charges. Brooks was not wearing a helmet.